Structural characterization of the ankyrin-repeat containing oncoprotein gankyrin in the presence of small molecules
The Tumor Ecosystem: Cellular Interactions and Therapeutic Opportunities
●
Bergamo, Italy
Sponsored by European Association for Cancer Research
Maryam Riyazi
,
Taylor Laflamme
,
Dipti Kanabar
,
T Chavan
,
Aaron Muth
,
Don Spratt
Apo-calmodulin binds to the HECT E3 ubiquitin ligase UBE3C via its N-terminal IQ motif
49th Lorne Conference on Protein Structure and Function
●
Lorne, Australia
Emily Schaffter
,
Megan Hill
,
Don Spratt
Structural impacts of small molecule drugs targeting the ankyrin repeat oncoprotein gankyrin
49th Lorne Conference on Protein Structure and Function
●
Lorne, Australia
Taylor Laflamme
,
Emma Kane
,
Maryam Riyazi
,
Dipti Kanabar
,
T Chavan
,
Aaron Muth
,
Don Spratt
Development of a kinetic FRET-based assay for the study of HECT E3 ligase inhibition by small molecules
49th Lorne Conference on Protein Structure and Function
●
Lorne, Australia
Amanda Brown
,
Don Spratt
New Discoveries on Protein Recruitment and Regulation during the Early Stages of the DNA Damage Response Pathways
International journal of molecular sciences
●
2024
●
Vol. 25
●
Issue #3
Kelly Waters
,
Donald Spratt
Synthesis and evaluation of 2,5-substituted pyrimidines as small-molecule gankyrin binders
Future medicinal chemistry
●
2024
●
Vol. 16
●
Issue #3
Dipti Kanabar
,
Emma Kane
,
Tejashri Chavan
,
Taylor Laflamme
,
Ethan Suarez
,
Mimansa Goyal
,
Vivek Gupta
,
Donald Spratt
,
Aaron Muth
ClarkNOW
Exchange broadening underlies underlies the enhancement of IDE-dependent degradation of insulin by anionic membranes
July
●
2022
●
Vol. 7
●
Issue #28
Q. Zheng
,
B. Lee
,
M. Kebede
,
V. Ivancic
,
M. Kemeh
,
H. Lemos Brito
,
Donald Spratt
,
Noel Lazo
Small molecule gankyrin inhibition as a therapeutic strategy for breast and lung cancer
Journal of Medicinal Chemistry
June
●
2022
●
Vol. 65
●
Issue #13
D. Kanabar
,
M. Goyal
,
Emma Kane
,
T. Chavan
,
A. Kabir
,
X. Wang
,
S. Shukla
,
J. Almasri
,
S. Goswami
,
G. Osman
,
M. Kokolis
,
V. Gupta
,
Donald Spratt
,
Aaron Muth
Redefining the Catalytic HECT Domain Boundaries for the HECT E3 Ubiquitin Ligase Family
September
●
2022
●
Vol. 42
●
Issue #10
Emma Kane
,
Steven Beasley
,
Johanna Schafer
,
Justine Bohl
,
Young Sun Lee
,
Kayla Rich
,
Elizabeth Bosia
,
Donald Spratt
Symposium session addresses Clark’s impact in the community: Off-campus connections transformative for students, professors
Intersection of Redox Chemistry and Ubiquitylation: Post-Translational Modifications Required for Maintaining Cellular Homeostasis and Neuroprotection
August
●
2021
●
Vol. 10
●
Issue #8
Emma Kane
,
Kelly Waters
,
Donald Spratt
Differential Effects of Polyphenols on Insulin Proteolysis by the Insulin-Degrading Enzyme
August
●
2021
●
Vol. 10
●
Issue #9
Qiuchen Zheng
,
Micheal Kebede
,
Bethany Lee
,
Cl Krasinski
,
Saadman Islam
,
Lili Wurfl
,
Merc M Kemeh
,
V Ivancic
,
Charl Jakobsche
,
Donald Spratt
,
Noel Lazo
HERC5 and the ISGylation Pathway: Critical Modulators of the Antiviral Immune Response
June
●
2021
●
Vol. 13
●
Issue #6
Nicholas Mathieu
,
Ermela Paparisto
,
Stephen Barr
,
Donald Spratt
2021 Lund Awards recognize service to the Worcester community: Recipients honored for work in STEM education, youth mentorship, addiction, and park restoration
ClarkU Worcester County High School STEM Careers Outreach (Online & In-person)
Clark students learn what’s cooking in the chemistry lab: Kitchen Chemistry course teaches the tasty basics of science
Exploring the Roles of HERC2 and the NEDD4L HECT E3 Ubiquitin Ligase Subfamily in p53 Signaling and the DNA Damage Response
Nicholas Mathieu
,
Rafael Levin
,
Donald Spratt
Structural Insights into Ankyrin Repeat-Containing Proteins and Their Influence in Ubiquitylation
International Journal of Molecular Sciences
January
●
2021
●
Vol. 22
●
Issue #2
Emma Kane
,
Donald Spratt
How ubiquitin determines the fate of our proteins
Five practical tips for a successful career in STEM
An Angelman syndrome substitution in the HECT E3 ubiquitin ligase C-terminal Lobe of E6AP affects protein stability and activity
July
●
2020
●
Vol. 15
●
Issue #7
Steven Beasley
,
Chloe Kellum
,
Rachel Orlomoski
,
Feston Idrizi
,
Donald Spratt
HECT E3 ubiquitin ligases - emerging insights into their biological roles and disease relevance
April
●
2020
●
Vol. 133
●
Issue #7
Yaya Wang
,
Diana Argiles-Castillo
,
Emma Kane
,
Anning Zhou
,
Donald Spratt
Published by IntechOpen
●
2020
Emma Kane
,
Donald Spratt
Crystal structure of the catalytic C-lobe of the HECT-type ubiquitin ligase E6AP
January
●
2020
●
Vol. 29
●
Issue #6
Lena Ries
,
Anna Liess
,
Christian Feiler
,
Donald Spratt
,
Edward Lowe
,
Sonja Lorenz
A subset of calcium-binding S100 proteins show preferential heterodimerization
January
●
2019
●
Vol. 286
●
Issue #10
Donald Spratt
,
Kathryn Barber
,
Nicole Marlatt
,
V Ngo
,
Jillian Macklin
,
Yiming Xiao
,
Lars Konermann
,
Martin Duennwald
,
Gary Shaw
Rapid and efficient purification of Drosophila homeodomain transcription factors for biophysical characterization
Protein Expression and Purification
February
●
2019
●
Vol. 158
Rachel Orlomoski
,
Aaron Bogle
,
Jeanmarie Loss
,
Rylee Simons
,
Jacqueline Dresch
,
Robert Drewell
,
Donald Spratt
The HECT C-terminal lobe of HERC6 reveals a unique autoinhibitory domain swapping mechanism
Young Investigator Talk, Protein Society
●
Boston MA
Sponsored by 32nd Protein Society Annual Symposium, Protein Society
Resveratrol sustains insulin-degrading enzyme activity toward Abeta42
October
●
2018
●
Vol. 3
●
Issue #10
Claire Krasinski
,
Valerie Ivancic
,
Qiuchen Zheng
,
Donald Spratt
,
Noel Lazo
Enzyme kinetics from circular dichroism of insulin reveals mechanistic insights into the regulation of insulin-degrading enzyme
November
●
2018
●
Vol. 38
●
Issue #6
Valerie Ivancic
,
Claire Krasinski
,
Qiuchen Zheng
,
Rebecca Meservier
,
Donald Spratt
,
Noel Lazo
1H, 13C, and 15N resonance assignments of the C-terminal lobe of the human HECT E3 ubiquitin ligase ITCH
Biomolecular NMR Assignments
September
●
2018
●
Vol. 13
●
Issue #1
Steven Beasley
,
Roela Bardhi
,
Donald Spratt
The longest amyloid-beta precursor protein intracellular domain produced with Abeta42 forms beta-sheet-containing monomers that self-assemble and are proteolyzed by insulin-degrading enzyme
ACS Chemical Neuroscience
August
●
2018
●
Vol. 9
●
Issue #12
Claire Krasinski
,
Qiuchen Zheng
,
Valerie Ivancic
,
Donald Spratt
,
Noel Lazo
Generation of phospho-ubiquitin variants by orthogonal translation reveals codon skipping
May
●
2016
●
Vol. 590
●
Issue #10
Susanna George
,
Jacob Aguirre
,
Donald Spratt
,
Yumin Bi
,
Madeline Jeffery
,
Gary Shaw
,
Patrick O'Donoghue
Suramin inhibits cullin-RING E3 ubiquitin ligases
Proceedings of the National Academy of Sciences
March
●
2016
●
Vol. 113
●
Issue #14
Kenneth Wu
,
Robert Chong
,
Qing Yu
,
Jin Bai
,
Donald Spratt
,
Kevin Ching
,
Chan Lee
,
Haibin Miao
,
Inger Tappin
,
Jerard Hurwitz
,
others
Disruption of the autoinhibited state primes the E3 ligase parkin for activation and catalysis
August
●
2015
●
Vol. 34
●
Issue #20
Atul Kumar
,
Jacob Aguirre
,
Tara Condos
,
R Martinez-Torres
,
Viduth Chaugule
,
Rachel Toth
,
Ramasubramanian Sundaramoorthy
,
Pascal Mercier
,
Axel Knebel
,
Donald Spratt
,
others
Pivotal role for the ubiquitin Y59-E51 loop in lysine 48 polyubiquitination
Proceedings of the National Academy of Sciences
June
●
2014
●
Vol. 111
●
Issue #23
Robert Chong
,
Kenneth Wu
,
Donald Spratt
,
Yingying Yang
,
Chan Lee
,
Jaladhi Nayak
,
Ming Xu
,
Rana Elkholi
,
Inger Tappin
,
Jessica Li
,
others
RBR E3 ubiquitin ligases: new structures, new insights, new questions
February
●
2014
●
Vol. 458
●
Issue #3
Donald Spratt
,
Helen Walden
,
Gary Shaw
A snapshot of ubiquitin chain elongation: lysine 48-tetra-ubiquitin slows down ubiquitination
Journal of Biological Chemistry
January
●
2014
●
Vol. 289
●
Issue #10
Jordan Kovacev
,
Kenneth Wu
,
Donald Spratt
,
Robert Chong
,
Chan Lee
,
Jaladhi Nayak
,
Gary Shaw
,
Zhen-Qiang Pan
Structure of the HHARI catalytic domain shows glimpses of a HECT E3 ligase
August
●
2013
●
Vol. 8
●
Issue #8
Donald Spratt
,
Pascal Mercier
,
Gary Shaw
A molecular explanation for the recessive nature of parkin-linked Parkinson's disease
June
●
2013
●
Vol. 4
●
Issue #1
Donald Spratt
,
R Martinez-Torres
,
Yeong Noh
,
Pascal Mercier
,
Noah Manczyk
,
Kathryn Barber
,
Jacob Aguirre
,
Lynn Burchell
,
Andrew Purkiss
,
Helen Walden
,
others
Structure and dynamics of calmodulin (CaM) bound to nitric oxide synthase peptides: effects of a phosphomimetic CaM mutation
April
●
2012
●
Vol. 51
●
Issue #17
Michael Piazza
,
Kathryn Futrega
,
Donald Spratt
,
Thorsten Dieckmann
,
J Guillemette
Selective recruitment of an E2~ubiquitin complex by an E3 ubiquitin ligase
Journal of Biological Chemistry
March
●
2012
●
Vol. 287
●
Issue #21
Donald Spratt
,
Kenneth Wu
,
Jordan Kovacev
,
Zhen-Qiang Pan
,
Gary Shaw
Expression and purification of an isotopically labeled aggregation prone inducible nitric oxide synthase calmodulin-binding protein for use in nuclear magnetic resonance studies
Journal of Labelled Compounds and Radiopharmaceuticals
April
●
2011
●
Vol. 54
●
Issue #10
Michael Piazza
,
Yay Duangkham
,
Donald Spratt
,
Thorsten Dieckmann
,
J Guillemette
Mapping the binding and calmodulin-dependent activation of nitric oxide synthase isozymes
The Open Nitric Oxide Journal
February
●
2011
●
Vol. 3
●
Issue #1
Spratt, E
,
Yay Duangkham
,
Valentina Taiakina
,
J Guillemette
Association of the disordered C-terminus of CDC34 with a catalytically bound ubiquitin
Journal of Molecular Biology
February
●
2011
●
Vol. 407
●
Issue #3
Donald Spratt
,
Gary Shaw
Codon optimization for enhanced Escherichia coli expression of human S100A11 and S100A1 proteins
Protein Expression and Purification
March
●
2010
●
Vol. 73
●
Issue #1
Nicole Marlatt
,
Donald Spratt
,
Gary Shaw
Intraprotein electron transfer in inducible nitric oxide synthase holoenzyme
Journal of Biological Inorganic Chemistry
October
●
2009
●
Vol. 14
●
Issue #1
Changjian Feng
,
Andrea Dupont
,
Nickolas Nahm
,
Donald Spratt
,
James Hazzard
,
J Weinberg
,
J Guillemette
,
Gordon Tollin
,
Dipak Ghosh
FRET conformational analysis of calmodulin binding to nitric oxide synthase peptides and enzymes
September
●
2008
●
Vol. 47
●
Issue #46
Donald Spratt
,
Valentina Taiakina
,
Michael Palmer
,
J Guillemette
Regulation of mammalian nitric oxide synthases by electrostatic interactions in the linker region of calmodulin
Biochimica et Biophysica Acta - Proteins and Proteomics
September
●
2008
●
Vol. 1784
●
Issue #12
Donald Spratt
,
Odisho Israel
,
Valentina Taiakina
,
J Guillemette
Calcium-deficient calmodulin binding and activation of neuronal and inducible nitric oxide synthases
Biochimica et Biophysica Acta - Proteins and Proteomics
August
●
2007
●
Vol. 1774
●
Issue #10
Donald Spratt
,
Valentina Taiakina
,
J Guillemette
Differential binding of calmodulin domains to constitutive and inducible nitric oxide synthase enzymes
February
●
2007
●
Vol. 46
●
Issue #28
Donald Spratt
,
Valentina Taiakina
,
Michael Palmer
,
J Guillemette
Selective labeling of selenomethionine residues in proteins with a fluorescent derivative of benzyl bromide
October
●
2006
●
Vol. 359
●
Issue #2
Shenhui Lang
,
Donald Spratt
,
J Guillemette
,
Michael Palmer
Binding and activation of nitric oxide synthase isozymes by calmodulin EF hand pairs
February
●
2006
●
Vol. 273
●
Issue #8
Donald Spratt
,
Elena Newman
,
Jennifer Mosher
,
Dipak Ghosh
,
John Salerno
,
J Guillemette
Dual-targeted labeling of proteins using cysteine and selenomethionine residues
May
●
2005
●
Vol. 342
●
Issue #2
Shenhui Lang
,
Donald Spratt
,
J Guillemette
,
Michael Palmer
Differential activation of nitric-oxide synthase isozymes by calmodulin-troponin C chimeras
Journal of Biological Chemistry
May
●
2004
●
Vol. 279
●
Issue #32
Elena Newman
,
Donald Spratt
,
Jennifer Mosher
,
B Cheyne
,
Heather Montgomery
,
Denney Wilson
,
J Weinberg
,
Susan Smith
,
John Salerno
,
Dipak Ghosh
,
others
Precise measurements of protein-DNA binding affinities support the use of PWM-based approaches in modeling transcriptional regulation
Jacqueline Dresch
,
Fadwa Mekkaoui
,
Eleanor Verhagen
,
Rachel Orlomoski
,
Sean Monroe
,
Aaron Bogle
,
Navid Al-Hassan
,
Robert Drewell
,
Don Spratt
